Krameria lappacea (Rhatany or Ratanhia, Krameriaceae) is an endangered, overexploited medicinal plant from the semi-deserts of Andean. Description and images of Krameria lappacea (), a native Chilean plant, provided by the supplier of native exotic Chilean seeds and organizer of plant watching. Krameria lappacea (Rhatany or Ratanhia, Krameriaceae) is an endangered, overexploited medicinal plant from the semi-deserts of Andean South America, and.
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Separation was possible by changing the stationary phase from the phenyl-hexyl material to a RP column material, but as highlighted before this made a separation of compounds 11 and 12 impossible. In order to check if this extraction protocol is exhaustive, another 5 cycles after the normal extraction protocol were conducted and the obtained extract analyzed by HPLC.
The methanol insoluble part Precision of the method was determined by preparing and assaying 5 replicate samples of RR-1 and T-1 on the same day; the same procedure was repeated on two more days.
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Attempts to improve the resolution with the aid of additives, such as tetrahydrofuran, 1- and 2-propanol or tert-butylmethyl ether, or a variation of temperature showed no effect. Repeatability of the method was assured by relative standard deviations of multiple injections.
Introduction Krameria lappacea Dombey Burdet et Simpson; syn. Maximum deviations within one day intra-day mrameria were 4. Conclusions In a previous study we could show that not only tannins with their astringent and antimicrobial properties, but also the neglected compound class of lignan derivatives with their pronounced anti-inflammatory activities play an important role in the use of the herbal drug against inflammatory disorders of mouth and throat.
Wikipedia articles incorporating a citation from the Encyclopaedia Britannica with Wikisource reference Articles with lappxcea microformats Wikipedia articles incorporating a citation from the Encyclopedia Americana with a Wikisource reference Commons category link from Wikidata. This page was last edited on 21 Novemberat From Wikipedia, the free encyclopedia. Known amounts of the standard compounds were added either to the dry powdered root material, followed by lappcea or were applied on the polyamide column together with the tincture.
The authors thank Ernst P. All other HPLC parameters lap;acea maintained. Abstract Root preparations of Krameria lappacea Dombey Burdet et Simpson are traditionally used against oropharyngeal inflammation.
Krameria lappacea – Useful Tropical Plants
Chromatograms of the standard mix, a typical root and a tincture sample under optimized conditions are depicted in Fig. The flowers have glands called elaiophores which produce a lipid which is collected by bees of the genus Centris as they pollinate the flowers. Retrieved from ” https: Suitability of the developed method for the quantification of the major lignan derivatives in Ratanhiae radix and Tinctura Ratanhiae can be deduced from several analytical parameters.
This article has been cited by other articles in PMC. The major problem in the development of a HPLC method for krmeria simultaneous detection of all active lignan derivatives was the structural similarity of the analytes.
Interestingly, root sample RR-1 showed a higher content of all benzofurans, except for compounds 6and Integrated Taxonomic Information System.
Associated Data Supplementary Materials mmc1. The composition of the mobile phase consisted of 0.
Solvent A was changed from 0. By comparing variations within the same days intra-day precisions were determined, by observing differences within the 3 days inter-day precision was deduced. They are perennial shrubs which act as root parasites on other plants.
Krameria are found across the Americaswith most native to the tropical regions. None of the compounds 1 — 12 could be quantified since their content was below the LOQ and even below the LOD, indicating an exhaustive extraction.
Compound 7 showed highest krrameria variance with maximum deviations of 2.
ESI, alternating mode; spray voltage: Moreover it was obvious that by using just methanol or acetonitrile alone no satisfactory separation was achieved. The root bark contains an almost insoluble free red substance called ratanhia red. Calibration and validation Individual stock solutions of standard compounds were prepared in methanol.
Standards Compounds 1 — 6and 8 — 12 were isolated and their structures elucidated in a previous study .